Human immune and gut microbial parameters associated with inter-individual variations in COVID-19 mRNA vaccine-induced immunity

COVID-19 mRNA vaccines induce protective adaptive immunity against SARS-CoV-2 in most individuals, but there is wide variation in levels of vaccine-induced antibody and T-cell responses. However, the mechanisms underlying this inter-individual variation remain unclear. Here, using a systems biology approach based on multi-omics analyses of human blood and stool samples, we identified several factors that are associated with COVID-19 vaccine-induced adaptive immune responses. BNT162b2-induced T cell response is positively associated with late monocyte responses and inversely associated with baseline mRNA expression of activation protein 1 (AP-1) transcription factors. Interestingly, the gut microbial fucose/rhamnose degradation pathway is positively correlated with mRNA expression of AP-1, as well as a gene encoding an enzyme producing prostaglandin E2 (PGE2), which promotes AP-1 expression, and inversely correlated with BNT162b2-induced T-cell responses. These results suggest that baseline AP-1 expression, which is affected by commensal microbial activity, is a negative correlate of BNT162b2-induced T-cell responses.journal articl

Event-related alpha suppression in response to facial motion

This article has been made available through the Brunel Open Access Publishing Fund.While biological motion refers to both face and body movements, little is known about the visual perception of facial motion. We therefore examined alpha wave suppression as a reduction in power is thought to reflect visual activity, in addition to attentional reorienting and memory processes. Nineteen neurologically healthy adults were tested on their ability to discriminate between successive facial motion captures. These animations exhibited both rigid and non-rigid facial motion, as well as speech expressions. The structural and surface appearance of these facial animations did not differ, thus participants decisions were based solely on differences in facial movements. Upright, orientation-inverted and luminance-inverted facial stimuli were compared. At occipital and parieto-occipital regions, upright facial motion evoked a transient increase in alpha which was then followed by a significant reduction. This finding is discussed in terms of neural efficiency, gating mechanisms and neural synchronization. Moreover, there was no difference in the amount of alpha suppression evoked by each facial stimulus at occipital regions, suggesting early visual processing remains unaffected by manipulation paradigms. However, upright facial motion evoked greater suppression at parieto-occipital sites, and did so in the shortest latency. Increased activity within this region may reflect higher attentional reorienting to natural facial motion but also involvement of areas associated with the visual control of body effectors. © 2014 Girges et al

Systematic analysis of mitochondrial genes associated with hearing loss in the Japanese population: dHPLC reveals a new candidate mutation

<p>Abstract</p> <p>Background</p> <p>Variants of mitochondrial DNA (mtDNA) have been evaluated for their association with hearing loss. Although ethnic background affects the spectrum of mtDNA variants, systematic mutational analysis of mtDNA in Japanese patients with hearing loss has not been reported.</p> <p>Methods</p> <p>Using denaturing high-performance liquid chromatography combined with direct sequencing and cloning-sequencing, Japanese patients with prelingual (N = 54) or postlingual (N = 80) sensorineural hearing loss not having pathogenic mutations of m.1555A > G and m.3243A > G nor <it>GJB2 </it>were subjected to mutational analysis of mtDNA genes (<it>12S rRNA</it>, <it>tRNA</it>^{<it>Leu(UUR)</it>}, <it>tRNA</it>^{<it>Ser(UCN)</it>}, <it>tRNA</it>^<it>Lys</it>, <it>tRNA</it>^<it>His</it>, <it>tRNA</it>^{<it>Ser(AGY)</it>}, and <it>tRNA</it>^<it>Glu</it>).</p> <p>Results</p> <p>We discovered 15 variants in <it>12S rRNA </it>and one homoplasmic m.7501A > G variant in <it>tRNA</it>^{<it>Ser(UCN)</it>}; no variants were detected in the other genes. Two criteria, namely the low frequency in the controls and the high conservation among animals, selected the m.904C > T and the m.1105T > C variants in <it>12S rRNA </it>as candidate pathogenic mutations. Alterations in the secondary structures of the two variant transcripts as well as that of m.7501A > G in <it>tRNA</it>^{<it>Ser(UCN) </it>}were predicted.</p> <p>Conclusions</p> <p>The m.904C > T variant was found to be a new candidate mutation associated with hearing loss. The m.1105T > C variant is unlikely to be pathogenic. The pathogenicity of the homoplasmic m.7501T > A variant awaits further study.</p

Aspen Growth Response in the Presence of Inter-Annual Climate Fluctuation and Disturbance in the Lake Tahoe Basin

In the western US, aspen forests tend to be small and rare, but have great ecological importance. There is much interest and concern over how aspen in the Sierra Nevada Mountains of the western USA will respond to a changing climate and future disturbances. Impacts from climate change create stress on aspen trees that further compound threats to aspen communities. This analysis assessed the radial growth response of aspen under previously recorded climate conditions to better understand how measurable climate variables affect aspen growth. Along with aspen’s growth response to climate, this analysis also assessed the growth response of aspen within the vicinity of a wildfire by measuring growth from aspen stands above and below the 2002 Showers fire footprint. Increment cores were collected from aspen trees in 20 stands around Lake Tahoe, California and Nevada, USA, spanning different aspects, elevations, and species compositions. Tree ring widths were measured using WinDENDRO and the data were visually cross-dated through microscopic comparison. The relationship between aspen growth, climate, disturbance, and stand conditions were analyzed using linear mixed effects regression. The models incorporated random effects for time and space since the data exhibited temporal and spatial autocorrelation. The data were separated into northeast (NE) and southwest (SW) regions of the Lake Tahoe Basin based on the similarities of the stands’ climate values revealed by a dendrogram. In both regions, the most influential climate variables were annual maximum temperature and annual precipitation. In the NE region, the highest aspen tree basal area increment (BAI) was measured in previously recorded years with a low temperature/high precipitation climate regime. For the SW region of the Lake Tahoe Basin, aspen tree BAI was higher under a low temperature/low precipitation climate regime. Along with climate, stand level variables such as canopy stratum (overstory/understory), elevation, and species composition (percent aspen presence) also influenced growth of aspen trees. The regression analysis indicated that aspen BAI was greater in areas with a higher proportion of aspen composition and for dominant trees in the canopy. However, aspen BAI declined with increases in elevation. The post-wildfire analysis modeled how aspen responded when downstream of a wildfire compared to unaffected stands upstream, where downstream aspen could be influenced by added availability of water and nutrients, due to increased runoff and erosion from the fire. However, only the stand closest to the burned area exhibited a significant increase in aspen tree growth downstream from the wildfire. A response was not detected when stands further downstream were included in the analysis. Therefore, a wildfire could produce increases in aspen growth post-disturbance depending on proximity to the fire. In terms of growth response longevity, increased growth was detected in ratios of growth over a 3- and 5-year period from when the fire occurred